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2 edition of Recognition of N-acetyl [gamma]-D-galactosamine by the seed lectin from Dolichos biflorus found in the catalog.

Recognition of N-acetyl [gamma]-D-galactosamine by the seed lectin from Dolichos biflorus

Roslyn Victoria Gibbs

Recognition of N-acetyl [gamma]-D-galactosamine by the seed lectin from Dolichos biflorus

by Roslyn Victoria Gibbs

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Published by Portsmouth Polytechnic, School of Pharmacy and Biomedical Sciences in Portsmouth .
Written in English


Edition Notes

Statementby Roslyn VictoriaGibbs.
ID Numbers
Open LibraryOL20565919M

  The lectins WGA (specific for GlcNAc and Neu5Ac), SNA, MAA, Achatinin-H (specific for 9-O-acetyl sialoglycosyl residue) were used to show the presence of sialic acids. Unbound radioactivity was removed using TBS-bovine serum albumin (1 mg/ml) and bound I-spectrin VL/N was measured by Gamma-counter (Electronic Corporation, India).Cited by: everything up until page of the book. If anyone wants to make a quizlet for the rest of it we can combine them. Search. Create. a. react with Dolichos biflorus b. bE-with anti-A c. five a mixed field reaction with anti-A,B c. they react with Arachis hypogaea lectin.

  The decidual cells, not only secrete growth factors but also release tissue inhibitor of matrix metalloproteinases (TIMPs) to suppress trophoblast-derived matrix metalloproteinases (MMPs) [35, 36] and express contact-dependent signaling molecules such as connexin 43 (Cx43) [].Although not all of the matrix proteins have been shown to be specifically expressed in either the human or murine Cited by:   The decidua has been known as maternal uterine tissue, which plays essential roles in protecting the embryo from being attacked by maternal immune cells and provides nutritional support for the developing embryo prior to placenta formation. However, there are questions that still remain to be answered: (1) How does the decidua supply nutrition and provide a physical scaffold for the growing Cited by:

Similarly, a root surface lectin of Dolichos biflorus has apyrase (Ca 2+-dependent ATP diphosphatase) activity and binds Nod factors from both heterologous and homologous rhizobia. Reverse PCR-based techniques using mRNAs of L. japonicus and M. sativa root confirmed the presence of orthologues in these two by:   Nodule formation induced by nitrogen-fixing rhizobia depends on bacterial nodulation factors ([NF][1]s), modified chitin oligosaccharides with a fatty acid moiety. Certain [NF][1]s can be cleaved and inactivated by plant chitinases. However, the most abundant [NF][1] of Sinorhizobium meliloti, an O -acetylated and sulfated tetramer, is resistant to hydrolysis by all plant chitinases tested so Cited by:


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Recognition of N-acetyl [gamma]-D-galactosamine by the seed lectin from Dolichos biflorus by Roslyn Victoria Gibbs Download PDF EPUB FB2

Two differentially expressed lectins from the legume Dolichos biflorus, the seed lectin and a stem and leaf lectin (DB58), were photoaffinity-labeled at their adenine binding sites using the probe.

The role of lectins in blood group serology. Judd WJ. Many lectins display blood group activity, and extracts from Dolichos biflorus (anti-A1), Ulex europaeus (anti-H), and Vicia graminea (anti-N) seeds provide an alternative to human sera as a source of blood-typing by:   Abstract.

The mannose/glucose-binding Dolichos lablab lectin (designated DLL) has been purified from seeds of Dolichos lablab (hyacinth bean) to electrophoretic homogeneity by affinity chromatography on an ovalbumin-Sepharose 4B column. The purified lectin gave a single symmetric protein peak with an apparent molecular mass of 67 kDa on gel filtration chromatography, and five Cited by: The in vivo biosynthesis of the Dolichos biflorus seed lectin was studied by pulse-chase labeling experiments using [(35)S] methionine and [(14)C]glucosamine.

Dolichos lablab plants belong to the family Leguminosae and are widely grown as a food seed of the lablab bean was reported to contain at least two different types of lectins: DLL-I, that displays affinity for glucose/mannose (Glc/Man) and DLL-II with affinity for galactose (Gal), sharing a sequence identity of ∼41%.The structure of FRIL (Flt3 receptor interacting lectin), another.

The structure of Dolichos biflorus lectin (DBL), another Gal‐binding lectin, also from Leguminosae family was reported earlier (Hamelryck et al. 14 PDB code: 1LU1) and has a sequence identity of 69% with DLL‐II DBL forms a soybean lectin (SBA) type of tetramer and is the first lectin for which a structural view of the adenine binding to Cited by: 7.

Previous studies have shown that the Dolichos biflorus plant contains a lectin in its stems and leaves, called DB58, that is closely related to the D. biflorus seed lectin. DB58 is a heterodimer. Dolichos biflorus agglutinin DBA GalNAc αGalNAc Major vessels 1 Griffonia simplicifolia Lectin I GS 1, isolectin B4 α-linked Gal All beds excepts those of kidney, liver or adrenal gland 1,2 Helix pomatia agglutinin HPA α linked terminal GalNAc All beds excepts those of kidney, liver or adrenal gland 1,2Cited by: Vol Issue 5, October ISSN: (Print) (KB) Lectin Papers.

A comparison of the carbohydrate binding properties of twoDolichos biflorus lectins. Marilynn E. Etzler Pages Lectin Papers. Molecular modelling of theDolichos biflorus seed lectin and its specific interactions with carbohydrates: α-D-N-acetyl.

The seeds contained only low levels of essentially non-toxic lectin, moderate amounts of trypsin inhibitors and negligible quantities of a-amylase inhibitors and they have great potential as.

A few galactose/N-acetylgalactosamine (Gal/GalNAc)-binding lectins isolated from legume plants have proven to be useful markers for cancer histochemistry, such as VVLB4 (Vicia villosa isolectin 4), PHA (Phaseolus vulgaris agglutinin), DBL (Dolichos biflorus lectin), SBA (Gycine max) and PNA (Arachis hypogaea), among others (Bakalova and Ohba Cited by: Abstract.

Expression of the binding properties of Gal/GalNAc specific lectins, based on the affinity of decreasing order of mammalian glycotopes (determinants) rather than saccharide inhibition pattern, is probably one of the best ways to express hydrate specifity and should facilitate the selection of lectins as structural probes for ng mammalian by: A.M.

Wu, E.A. Rabat, F.G. Gruezo, and H.J. Allen, Immunochemical studies on the combining site of the D - galactopyranose and 2-acetamidodeoxy-D-galactopyranose specific lectin isolated from Bauhinia purpurea alba seeds.

by: Maackia amurensis lectins ex- tracted from M. amurensis seed extracts contain a mixture of MAL I and Mal II lectins (Varki and Varki, ). Even though both are structu- rally similar, MAL I and. The lectin present in horse gram (Dolichos biflorus) seeds is a blood-group-A-specific lectin.

Bird [] first reported seed extracts agglutinated human type A 1 erythrocytes fold more strongly than A 2 cells. Purified by affinity chromatography on immobilized polyleucyl hog A + H substance [], the Dolichos biflorus lectin consists of a. Sandra Lyn Biroc and Marilyn E.

Etzler, The effect of periodate oxidation and α-mannosidase treatment of Dolichos biflorus lectin, Biochimica et Biophysica Acta (BBA) - General Subjects, /(78)X,1, (), ().Cited by:   The lectin-like receptor kinases (LecRKs) are a class of proteins originally described from Arabidopsis (Hervé et al., ).They have a structure similar to other plant receptor-like kinases (RLKs; Shiu and Bleecker, ; Cock et al., ) with an N-terminal targeting signal, a presumably extracellular domain, a single transmembrane (TM)-spanning helix, and a cytosolic kinase by: Some of the glycolipids and glycoproteins on an individual’s red blood cells can be identified by lectins.

A lectin from Dolichos biflorus is used to identify cells that belong to the A1 blood group. A lectin from Ulex europaeus is used to identify the H blood group antigen.

A lectin from Vicia graminea is used to identify the N blood group. Etzler, M. E., and Kabat, E.Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity from Dolichos biflorus, Biochemistry PubMed Google ScholarCited by: 4.

Rosenberg DW. Non-homogeneous marking of distal colonic mucosa using Dolichos. biflorus lectin. Cancer Lett. Nov 27;98(1) PubMed PMID: view Abstract. Soudais C, Bielinska M, Heikinheimo M, MacArthur CA, Narita N, Saffitz JE, Simon MC, Leiden JM, Wilson DB. Targeted mutagenesis of the transcription factor.

The lectins WGA (specific for GlcNAc and Neu5Ac), SNA, MAA, Achatinin-H (specific for 9-O-acetyl sialoglycosyl residue) were used to show the presence of sialic acids. Unbound radioactivity was removed using TBS-bovine serum albumin (1 mg/ml) and bound I-spectrin VL/N was measured by Gamma-counter (Electronic Corporation, India) [47].Cited by: c (Dolichos biflorus plant seed extract forms complexes with N-acetylgalactosamine.

When properly diluted, it can distinguish between A1 donor cells and all other subgroups of .The epithelial-mesenchymal transition (EMT) is an essential step in the proliferation and metastasis of solid tumor cells, and glycosylation plays a crucial role in the EMT process.

Certain aberrant glycans have been reported as biomarkers during bladder cancer progression, but global variation of N-glycans in this type of cancer has not been previously by: